Quantitative whole
body autoradiography
of a rat killed 2 h after
intravenous
administration of a
radioiodinated
cannabinoid drug.
Accumulation of
radioactivity in the brain
reflects binding to
cannabinoid receptors,
with high levels in the
cerebellum,
hippocampus and
globus pallidus. High
uptake is also apparent
in the liver, intestine,
harderian gland and
adipose tissue, the
latter reflecting the high
lipophilicity of this
compound
body autoradiography
of a rat killed 2 h after
intravenous
administration of a
radioiodinated
cannabinoid drug.
Accumulation of
radioactivity in the brain
reflects binding to
cannabinoid receptors,
with high levels in the
cerebellum,
hippocampus and
globus pallidus. High
uptake is also apparent
in the liver, intestine,
harderian gland and
adipose tissue, the
latter reflecting the high
lipophilicity of this
compound
Single organ autoradiography (brain) in a rat
killed 30 min after intravenous administration of a
radioiodinated cannabinoid receptor agonist.
Brain sections cut in a sagittal orientation.
Accumulation of radioactivity in the brain
parallels the distribution of cannabinoid
receptors, with high levels in the cerebellum,
hippocampus and globus pallidus.
killed 30 min after intravenous administration of a
radioiodinated cannabinoid receptor agonist.
Brain sections cut in a sagittal orientation.
Accumulation of radioactivity in the brain
parallels the distribution of cannabinoid
receptors, with high levels in the cerebellum,
hippocampus and globus pallidus.
Quantitative whole
body autoradiography
and adjacent
photographed section
of a rat killed 1 h after
intravenous
administration of
20µCi
[14C]2-deoxyglucose.
High uptake is
observed in the
harderian and
salivary glands and in
the brain, intestinal
lining and bone
marrow. These latter
areas reflect regions
with high metabolic
demand.
body autoradiography
and adjacent
photographed section
of a rat killed 1 h after
intravenous
administration of
20µCi
[14C]2-deoxyglucose.
High uptake is
observed in the
harderian and
salivary glands and in
the brain, intestinal
lining and bone
marrow. These latter
areas reflect regions
with high metabolic
demand.
Whole-body
autoradiography
(qwba) and adjacent
photographed section
of a rat killed 1 h after
intravenous
administration of 40 µCi
[14C]-ethanol. High
uptake is observed in
pancreatic tissue,
possibly correlating
with the high incidence
of pancreatic disease
in alcoholism. High
uptake is also seen in
the harderian and
salivary glands and
liver and kidney. High
activity in the lumen of
the small intestine
represents hepatobilary
excretion.
autoradiography
(qwba) and adjacent
photographed section
of a rat killed 1 h after
intravenous
administration of 40 µCi
[14C]-ethanol. High
uptake is observed in
pancreatic tissue,
possibly correlating
with the high incidence
of pancreatic disease
in alcoholism. High
uptake is also seen in
the harderian and
salivary glands and
liver and kidney. High
activity in the lumen of
the small intestine
represents hepatobilary
excretion.
Whole body autoradiography of a
rat killed 2 min after intravenous
administration of [3H]cocaine.
High uptake is observed in the
brain and heart myocardium,
consistent with the rapid onset of
euphoria, tachycardia and
generalized sympathomimetic
response following intravenous
administration of cocaine.
rat killed 2 min after intravenous
administration of [3H]cocaine.
High uptake is observed in the
brain and heart myocardium,
consistent with the rapid onset of
euphoria, tachycardia and
generalized sympathomimetic
response following intravenous
administration of cocaine.
Quantitative whole-body autoradiography; QWBA
Overview: Whole body autoradiography produces an image of the distribution of a radioactively-labeled test drug over
the entire animal section.
Whole body autoradiography protocol: Administer radiolabeled test drug and snap freeze animal (or a dissected
organ) in hexane-dry ice slurry. Cut frozen sections on whole-body cryosectioner using a proprietary process. Image
radioactivity in at least 5 representative sections cut at 5 mm intervals. Also, photograph adjacent sections for
anatomical reference. Quantify radioactivity levels in individual tissues and express results as ng drug equivalent per
g tissue. Optionally analyse radioactivity in tissue punches by reverse phase HPLC.
Recommended radioactivity dose: 14C and 125I >50 µCi/kg; 3H ~ 1,000 µCi/kg
Suggested testing: 3 sacrifice time points (e.g. 1 h, 4 h, 24 h) with one male and one female animal per time point.
Approximate prices: whole body (or organ) autoradiography*
Typical setup fee: $ 3,000
$ 2,000/animal (14C, 125I) or $ 4,000/animal (3H)
Overview: Whole body autoradiography produces an image of the distribution of a radioactively-labeled test drug over
the entire animal section.
Whole body autoradiography protocol: Administer radiolabeled test drug and snap freeze animal (or a dissected
organ) in hexane-dry ice slurry. Cut frozen sections on whole-body cryosectioner using a proprietary process. Image
radioactivity in at least 5 representative sections cut at 5 mm intervals. Also, photograph adjacent sections for
anatomical reference. Quantify radioactivity levels in individual tissues and express results as ng drug equivalent per
g tissue. Optionally analyse radioactivity in tissue punches by reverse phase HPLC.
Recommended radioactivity dose: 14C and 125I >50 µCi/kg; 3H ~ 1,000 µCi/kg
Suggested testing: 3 sacrifice time points (e.g. 1 h, 4 h, 24 h) with one male and one female animal per time point.
Approximate prices: whole body (or organ) autoradiography*
Typical setup fee: $ 3,000
$ 2,000/animal (14C, 125I) or $ 4,000/animal (3H)
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Preclinical contract research services
InvivoPharm: In vivo autoradiography and
biodistribution CRO services
biodistribution CRO services
Images © 2007, 2008 Invivopharm Inc.