InvivoPharm - Whole Body Autoradiography Service

Services:

Quantitative Whole-Body Autoradiography; QWBA

Overview: Whole body autoradiography produces an image of the distribution of a radioactively-labeled drug over the entire animal section.

Whole body autoradiography protocol: Administer radiolabeled test drug, euthanize and snap freeze animal (or a dissected organ) in hexane-dry ice slurry. Cut frozen sections and image radioactivity in at least 4 representative sections cut at 5 mm intervals, together with blood standards. Photograph adjacent sections for anatomical reference. Optionally, quantify radioactivity levels in individual structures and express results as ng drug equivalent per g tissue.

Recommended radioactivity dose: ¹⁴C and ¹²⁵I 50 uCi/kg; ³H ~ 1,000 uCi/kg

Suggested testing: 3 sacrifice time points (e.g. 1 h, 4 h, 24 h) with one male and one female animal per time point

Examples:

Quantitative whole body autoradiography and adjacent photographed section of a rat killed 1 h after intravenous administration of 20 uCi [14C]2-deoxyglucose. High uptake is observed in the harderian and salivary glands and in the brain, intestinal lining and bone marrow. These latter areas reflect regions with high metabolic demand.

Whole-body autoradiography (qwba) and adjacent photographed section of a rat killed 1 h after intravenous administration of 40 uCi [14C]-ethanol. High uptake is observed in pancreatic tissue, possibly correlating with the high incidence of pancreatic disease in alcoholism. High uptake is also seen in the harderian and salivary glands and liver and kidney. High activity in the lumen of the small intestine represents hepatobilary excretion.

Biodistribution of 125I-labeled carbon nanotubes at 2 h following intratracheal adminstration. Phosphor image in upper panel and adjacent photographed section in the lower panel. Nanotubes are mostly concentrated in the lung and intestine

Single organ autoradiography (brain) in a rat killed 30 min after intravenous administration of a radioiodinated cannabinoid receptor agonist. Brain sections cut in a sagittal orientation. Radioactivity distribution on the phosphor image parallels the distribution of cannabinoid receptors, with high levels in the cerebellum, hippocampus and globus pallidus

Rat Anatomical Guide for Whole Body Imaging

Radioiodination Service for Peptides and Proteins

InvivoPharm Inc: Whole body autoradiography CRO service