Sectioning technology and HPLC analysis of tissue radioactivity
Our whole-body sectioning machine cuts using a proprietary cutting process, developed in the labs of the co-founders, that allow us to cut
sections over a wide variety of thicknesses. This allows the option of taking tissue punches from frozen thick sections from regions of
interest identified in the corresponding autoradiographic sections. These tissue punches can then be used for subsequent HPLC analysis
of the radioactivity. Our proprietary cutting process additionally enables rapid sectioning through the entire animal carcass, reducing
sectioning time to as little as 30 min per animal and enabling us to keep our service prices low.
Example:
Our whole-body sectioning machine cuts using a proprietary cutting process, developed in the labs of the co-founders, that allow us to cut
sections over a wide variety of thicknesses. This allows the option of taking tissue punches from frozen thick sections from regions of
interest identified in the corresponding autoradiographic sections. These tissue punches can then be used for subsequent HPLC analysis
of the radioactivity. Our proprietary cutting process additionally enables rapid sectioning through the entire animal carcass, reducing
sectioning time to as little as 30 min per animal and enabling us to keep our service prices low.
Example:
Tissue punches taken from a frozen thick
section for analysis of tissue radioactivity
by HPLC. Rat had been injected with 20 uCi
14C-ethanol i.v. and sacrificed 1 h later.
Corresponding autoradiographic section is
shown below.
section for analysis of tissue radioactivity
by HPLC. Rat had been injected with 20 uCi
14C-ethanol i.v. and sacrificed 1 h later.
Corresponding autoradiographic section is
shown below.
Radiochromatogram of tissue radioactivity extracted from the brain of a rat that had
been injected with a radioiodinated cannabinoid drug. Retention time corresponded
to that of the unlabeled drug, indicating either little metabolism of this compound or an
exclusion of labeled metabolites by the blood-brain barrier
been injected with a radioiodinated cannabinoid drug. Retention time corresponded
to that of the unlabeled drug, indicating either little metabolism of this compound or an
exclusion of labeled metabolites by the blood-brain barrier
 | |  | |  |
Preclinical contract research services
