Biodistribution: Tissue dissection and counting
Overview: Determines the tissue distribution of a radioactively labeled test drug following systemic administration.
Radioactivity is determined by direct counting of individual tissues dissected from the animal.
Protocol summary: Administer radioactive test drug to animal (1 µCi/animal; oral/i.p./s.c./i.v.). Sacrifice animal at
various time points after injection. Collect blood sample and dissect out individual tissues. Weigh and digest tissues.
Count plasma and tissues in scintillation counter. Plot results as % injected dose/g tissue or as µg drug equivalent/g
tissue. Calculate Cmax, t1/2 and AUC for drug exposure by each organ.
Assay options:
Option 1: Count plasma and multiple organs/tissues (brain, heart, lung, liver, stomach, small intestine, large intestine,
kidneys, spleen, muscle, pancreas, skin, adrenal glands, bladder, testes etc.).
Option 2: Count plasma and individual brain structures (cortex, striatum, hippocampus, thalamus, cerebellum, brain
stem).
HPLC radio-analysis of tissue or plasma extracts
Radiodiodination service for peptides and proteins
Suggested testing: 6 sacrifice time points (e.g. 15 min, 30 min, 1 h, 2 h, 4 h, 24 h). n = 6 - 8 animals per time point.
Approximate prices*:
Typical setup fee: $ 3,000
Mice: $ 300/animal
Rat: $ 400/animal
*Please contact us to obtain a final quote and turn-around time.
Example data:
Overview: Determines the tissue distribution of a radioactively labeled test drug following systemic administration.
Radioactivity is determined by direct counting of individual tissues dissected from the animal.
Protocol summary: Administer radioactive test drug to animal (1 µCi/animal; oral/i.p./s.c./i.v.). Sacrifice animal at
various time points after injection. Collect blood sample and dissect out individual tissues. Weigh and digest tissues.
Count plasma and tissues in scintillation counter. Plot results as % injected dose/g tissue or as µg drug equivalent/g
tissue. Calculate Cmax, t1/2 and AUC for drug exposure by each organ.
Assay options:
Option 1: Count plasma and multiple organs/tissues (brain, heart, lung, liver, stomach, small intestine, large intestine,
kidneys, spleen, muscle, pancreas, skin, adrenal glands, bladder, testes etc.).
Option 2: Count plasma and individual brain structures (cortex, striatum, hippocampus, thalamus, cerebellum, brain
stem).
HPLC radio-analysis of tissue or plasma extracts
Radiodiodination service for peptides and proteins
Suggested testing: 6 sacrifice time points (e.g. 15 min, 30 min, 1 h, 2 h, 4 h, 24 h). n = 6 - 8 animals per time point.
Approximate prices*:
Typical setup fee: $ 3,000
Mice: $ 300/animal
Rat: $ 400/animal
*Please contact us to obtain a final quote and turn-around time.
Example data:
Mice injected i.v. with the radiolabeled drug and
sacrificed 1 - 8 h later. Data are means ± S.D. of 6
animals per time point. Moderate to good brain
uptake of the radiolabeled drug was apparent (2.5 %
ID/g @ 1 h).
sacrificed 1 - 8 h later. Data are means ± S.D. of 6
animals per time point. Moderate to good brain
uptake of the radiolabeled drug was apparent (2.5 %
ID/g @ 1 h).
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Preclinical contract research services
InvivoPharm: Drug distribution CRO service