Autoradiography in mouse brain with a radio-iodinated
cannabinoid CB1 receptor radioligand. Sections
(coronal) were imaged using phosphor imaging.
Radioactivity distribution is consistent with selective
binding to the CB1 receptor.
cannabinoid CB1 receptor radioligand. Sections
(coronal) were imaged using phosphor imaging.
Radioactivity distribution is consistent with selective
binding to the CB1 receptor.
Autoradiography in vitro (quantitative)
Overview: Determines the distribution and density of receptors in the brain or other target organ by incubation of
tissue sections in a radioactive drug.
Autoradiography protocol - distribution studies: Incubate cryostat-cut sections of brain or target organ in binding
buffer containing the radiolabeled drug until equilibrium is reached. Wash sections in fresh buffer to remove unbound
or loosely bound radiotracer. Optimal incubation conditions and wash time are determined in preliminary
experiments. Dry sections and image radioactivity using either phosphor imaging (125I, 3H, exposure time 1 -4 days)
or beta imaging (3H; exposure time 12 h; subcontracted).
Autoradiography protocol - competition studies: Incubate cryostat-cut sections of brain or target organ in binding
buffer containing a standard radiotracer for the receptor of interest along with various concentrations of the competing
unlabeled test drug. Wash sections in fresh buffer to remove unbound or loosely bound radiotracer. Dry sections and
image radioactivity. Draw regions-of-interest around selected brain or tissue structures and plot displacement curves
to obtain the drug IC50 for each region.
Suggested testing: For autoradiography distribution studies, 10 representative sections for the radiolabeled drug
alone and with an equivalent number of sections to define non-specific binding by co-incubation in a displacing
compound.
For competition studies, 5 representative sections incubated in 5 different concentrations of the competing drug
together with a vehicle control and reference displacing compound to define non-specific binding (= 35 sections total)
Approximate prices (distribution autoradiography protocol as per suggested testing)*:
Typical setup fee: $ 3,000 + radioligand and phosphor screen costs (where applicable)
$ 1,500 per brain or organ
*Please contact us to obtain a final quote and turn-around time. Setup fee reduced for larger or repeat contracts
Overview: Determines the distribution and density of receptors in the brain or other target organ by incubation of
tissue sections in a radioactive drug.
Autoradiography protocol - distribution studies: Incubate cryostat-cut sections of brain or target organ in binding
buffer containing the radiolabeled drug until equilibrium is reached. Wash sections in fresh buffer to remove unbound
or loosely bound radiotracer. Optimal incubation conditions and wash time are determined in preliminary
experiments. Dry sections and image radioactivity using either phosphor imaging (125I, 3H, exposure time 1 -4 days)
or beta imaging (3H; exposure time 12 h; subcontracted).
Autoradiography protocol - competition studies: Incubate cryostat-cut sections of brain or target organ in binding
buffer containing a standard radiotracer for the receptor of interest along with various concentrations of the competing
unlabeled test drug. Wash sections in fresh buffer to remove unbound or loosely bound radiotracer. Dry sections and
image radioactivity. Draw regions-of-interest around selected brain or tissue structures and plot displacement curves
to obtain the drug IC50 for each region.
Suggested testing: For autoradiography distribution studies, 10 representative sections for the radiolabeled drug
alone and with an equivalent number of sections to define non-specific binding by co-incubation in a displacing
compound.
For competition studies, 5 representative sections incubated in 5 different concentrations of the competing drug
together with a vehicle control and reference displacing compound to define non-specific binding (= 35 sections total)
Approximate prices (distribution autoradiography protocol as per suggested testing)*:
Typical setup fee: $ 3,000 + radioligand and phosphor screen costs (where applicable)
$ 1,500 per brain or organ
*Please contact us to obtain a final quote and turn-around time. Setup fee reduced for larger or repeat contracts
 | |  | |  |
Preclinical contract research services
InvivoPharm: In vitro autoradiography CRO service
Images © 2007, 2008 InvivoPharm Inc.